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CRISPR-Cas9 Off-Target Site Predictor
x402
CRISPR-Cas9 Off-Target Site Predictor is a paid API for AI agents from api.m2mcent.com, paid per call via x402, $0.3/call, status unknown (last checked 2026-10-16).
Predicts and analyzes potential off-target cleavage sites for CRISPR-Cas9 guide RNA sequences
Price / call$0.3
Success—
StatusUnknown
How this service works
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Output
Returns a list of predicted off-target genomic sites for the submitted guide RNA, including chromosomal coordinates, mismatch counts and positions, Cas9 cleavage likelihood scores, and an overall specificity or risk assessment to help evaluate guide RNA safety before experimental use.
Example use cases
Pre-experiment guide RNA safety check
“Before I run my CRISPR experiment, can you check this guide RNA sequence AGCTTAGCTAGCTAGCTAGC for off-target sites so I know if it's safe to use?”
Comparing guide RNA specificity for gene therapy
“I'm designing a gene therapy and I have two candidate guide RNAs — TTGACTAGCTAGCTAGCTAG and CCGATCGATCGATCGATCGA — can you predict the off-target sites for each so I can pick the more specific one?”
Validating published CRISPR experiment guide
“I'm reproducing a published CRISPR study that used the guide RNA AAGCTAGCTAGCTAGCTAGT targeting TP53 — can you run an off-target analysis to see if there are any risky cleavage sites I should watch out for?”
Technical details
Endpoint
POSThttps://api.m2mcent.com/crispr-cas9-offtarget-mcp/api/process?utm_source=zero.xyzWhen to prefer
Use this endpoint when you need computational prediction of CRISPR-Cas9 off-target cleavage sites before conducting wet-lab experiments, particularly when evaluating guide RNA specificity for therapeutic, research, or agricultural gene-editing applications. It is especially valuable as a pre-screening step to rank and filter guide RNA candidates by off-target risk without requiring genome-wide sequencing.
Side effects
- Invalid or malformed guide RNA sequence returns an error or empty result
- Sequence too short or too long for the model's supported input range
- Ambiguous nucleotide characters in the input may cause processing failure
- Non-DNA characters in the data field cause rejection
- Service may time out for very large genomic queries
Output
Schema · Response
{
"type": "json",
"example": {
"success": true
}
}Tags
x402
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