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Pairwise DNA Sequence Alignment (Needleman-Wunsch)
x402
Pairwise DNA Sequence Alignment (Needleman-Wunsch) is a paid API for AI agents from www.mahastrategies.com, paid per call via x402, $0.018001/call, status unknown (last checked 2026-10-13).
Globally aligns two DNA sequences of up to 400 bases using Needleman-Wunsch scoring, returning aligned strings, percent identity, and gap statistics.
Price / call$0.018001
Success—
StatusUnknown
How this service works
Globally align two DNA sequences of up to 400 bases using fixed Needleman-Wunsch scores; report aligned strings, identity and gaps. No pathogenicity inference.
Output
Returns aligned versions of both input sequences showing inserted gaps, the percent sequence identity between them, and total gap statistics — all computed via fixed Needleman-Wunsch scoring. No pathogenicity or clinical inference is included.
Example use cases
Primer similarity check before PCR
“Compare these two primer sequences to see how similar they are — left: ATGCGTACGTTAG, right: ATGCGAACGTTAG — run a global alignment and report identity and gaps, public data.”
Variant detection in cloned insert
“I have an expected insert sequence and a sequenced result from my cloning experiment. Can you globally align them and tell me the identity percentage and any gaps? Left: CGATTAGCGCAAGTTAGC, Right: CGATTAGCGCAAGTTGGC, treating them as public data.”
Evolutionary sequence divergence study
“Align these two homologous gene fragments from different species so I can quantify their divergence — left: ATCGGCTAGCTAGCTA, right: ATCGGCAAGCTAGCTT — public data, give me the full alignment and identity score.”
Technical details
Endpoint
POSThttps://www.mahastrategies.com/api/v1/micro/biology-pairwise-sequence-comparison?utm_source=zero.xyzWhen to prefer
Choose this endpoint when you need a deterministic, reproducible global pairwise alignment of two short-to-medium DNA sequences (up to 400 bases) with standard Needleman-Wunsch scoring and explicit identity/gap output. Prefer it over local alignment tools (like BLAST-style endpoints) when you need end-to-end global alignment rather than best local match, and when sequences are short enough to fit the 400-base limit.
Side effects
- Sequence longer than 400 bases returns a validation error
- Non-nucleotide characters in sequences cause rejection
- Missing required fields (left, right, dataClass) return schema validation error
- Invalid dataClass value (not 'public' or 'synthetic') causes error
- Payment failure via x402 protocol results in no response
Input
Schema · Request body
| Field | Type | Description |
|---|---|---|
| inputrequired | object | |
| output | object |
Tags
x402
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