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Pairwise DNA Sequence Comparison (Needleman-Wunsch)
x402
Pairwise DNA Sequence Comparison (Needleman-Wunsch) is a paid API for AI agents from www.mahastrategies.com, paid per call via x402, $0.035013/call, status unknown (last checked 2026-11-02).
Globally aligns two DNA sequences (up to 400 bases each) using Needleman-Wunsch scoring and returns aligned strings, percent identity, and gap counts.
Price / call$0.035013
Success—
StatusUnknown
How this service works
Globally align two DNA sequences of up to 400 bases using fixed Needleman-Wunsch scores; report aligned strings, identity and gaps. No pathogenicity inference. New modular adapter: retained result recovery for 24 hours, minimal receipt metadata for 90 days. Existing live route unchanged.
Output
Returns the two aligned sequence strings (with gap characters inserted), percent sequence identity, total gap count, and an alignment score computed via fixed Needleman-Wunsch parameters. Results are retained for retrieval for 24 hours; minimal receipt metadata is kept for 90 days.
Example use cases
Primer similarity check for lab design
“I have two candidate primer sequences — ATCGATCGATCGTTAA and ATCGATCGATCGTTCC — can you align them globally and tell me how similar they are and where the gaps fall? They're synthetic sequences.”
Mutation detection between two alleles
“Here are two 80-base DNA sequences from what might be two alleles of the same gene. Align them using global alignment and report the identity percentage and any gaps — AGCTTAGCTAGCTAGCTAAGCT... vs AGCTTAGCTAGCTTGCTAAGCT... Mark them as public data.”
Validation of synthetic sequence generation
“I generated a synthetic DNA sequence to match a target — can you align my generated sequence against the target and show me how well they match, including percent identity and number of gaps? Both are synthetic.”
Technical details
Endpoint
POSThttps://www.mahastrategies.com/api/v1/compute/biology/pairwise-sequence-comparison?utm_source=zero.xyzWhen to prefer
Choose this endpoint when you need a deterministic, fixed-scoring global Needleman-Wunsch alignment for two short DNA sequences (≤400 bases each) and require aligned strings with identity and gap statistics. Prefer it over local alignment (Smith-Waterman) when you want end-to-end global coverage. It does not infer pathogenicity or functional consequences, so it is appropriate for pure structural sequence comparison tasks on public or synthetic data.
Side effects
- Sequence exceeds 400-base limit — request rejected with validation error
- Invalid characters in sequence string (non-ACGT) — schema validation failure
- Unsupported dataClass value — enum validation error
- Network or payment failure — no alignment returned, receipt may still be issued
- Empty or missing left/right field — required-field validation error
Input
Schema · Request body
| Field | Type | Description |
|---|---|---|
| left | string | |
| right | string | |
| dataClass | string |
Tags
x402
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