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qPCR Run Quality Check
x402
qPCR Run Quality Check is a paid API for AI agents from www.mahastrategies.com, paid per call via x402, $0.010042/call, status unknown (last checked 2026-10-28).
Validates qPCR run data (Ct replicates, amplification efficiency, and control flags) against fixed research QC thresholds and reports Ct standard deviation plus any failed conditions.
Price / call$0.010042
Success—
StatusUnknown
How this service works
Check supplied Ct replicates, efficiency and control declarations against fixed research QC bands; report Ct SD and failed conditions. No diagnostic validation. New modular adapter: retained result recovery for 24 hours, minimal receipt metadata for 90 days. Existing live route unchanged.
Output
Returns the computed Ct standard deviation across the supplied replicates, a list of specific QC conditions that failed (e.g. high Ct SD, out-of-range efficiency, failed controls), and an overall QC pass/fail indication. Results are retained for 24 hours; minimal receipt metadata is stored for 90 days.
Example use cases
Gene expression replicate QC
“I have qPCR Ct replicates of [28.1, 28.4, 28.0, 28.7] from a gene expression experiment with 97% amplification efficiency, a passing no-template control, a single melt peak, and a stable reference gene — can you check whether these replicates meet research QC standards and tell me what failed?”
Screen for failed NTC control
“Check my qPCR run quality: Ct values are [30.2, 30.5, 30.1], efficiency is 85%, but I did not observe a clean no-template negative control — noTemplateNegative is false. The no-RT control passed and the melt curve shows a single peak. Flag any QC failures for me.”
Low-efficiency run assessment
“I want to know if my qPCR run passes QC — the Ct replicates are [19.8, 20.1, 19.9], but the amplification efficiency is only 60%, both negative controls passed, reference gene is stable, and I see a single melt peak. Data class is public.”
Technical details
Endpoint
POSThttps://www.mahastrategies.com/api/v1/compute/biology/qpcr-run-quality?utm_source=zero.xyzWhen to prefer
Use this endpoint when you need automated, rule-based QC assessment of a qPCR run against fixed research thresholds — particularly when you want a structured report of Ct SD and specific failed conditions without needing a clinical diagnostic validation framework. Prefer this over manual spreadsheet checks or general statistics endpoints when the input is specifically Ct replicate arrays combined with efficiency and control metadata.
Side effects
- Fewer than 1 or more than 128 Ct values supplied — validation error
- Efficiency value outside the acceptable numeric range — rejected input
- Missing required fields such as dataClass — schema validation failure
- Ct values with extreme outliers may produce high SD flags that are not indicative of a true run failure
- Ambiguous control states (e.g. null boolean flags) may be treated as absent rather than passed
Input
Schema · Request body
| Field | Type | Description |
|---|---|---|
| ct | array | |
| dataClass | string | |
| efficiency | number | |
| noRTNegative | boolean | |
| meltSinglePeak | boolean | |
| referenceStable | boolean | |
| noTemplateNegative | boolean |
Tags
x402
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